Peer-reviewed Research Papers

Review Articles

Book Chapters

Peer-Reviewed Research Papers

1. Q. Zhou, C. Wu, D. S. Manickam, D. Oupicky. “Evaluation of pharmacokinetics of bioreducible gene delivery vectors by real-time PCR.” Pharmaceutical Research, 2009, 26(7), 1581-1589. (Abstract)
2. Q. H. Zhou, Y. Z. You, C. Wu, Y. Huang, D. Oupicky. “Cyclic RGD-targeted reversibly-stabilized nanoparticles enhance cell uptake and transfection in vitro.” Journal of Drug Targeting, 2009, 17(5), 364-373. (Abstract)
3. J. Blacklock, Y. Z. You, Q. H. Zhou, G. Mao, D. Oupicky. “Gene delivery in vitro and in vivo from bioreducible multilayered polyelectrolyte films of plasmid DNA.” Biomaterials, 2009, 30, 939-950. (Abstract)
   
4. S. Kakade, D. S. Manickam, H. Handa, G. Mao, D. Oupicky. “Transfection activity of layer-by-layer plasmid DNA/poly(ethylenimine) films deposited on PLGA microparticles.” International Journal of Pharmaceutics, 2009, 365, 44-52. (Abstract)
5. L. Wan, D. S. Manickam, D. Oupicky, G. Mao. "DNA Release Dynamics from Reducible Polyplexes by Atomic Force Microscopy." Langmuir, 2008, 24, 12474-12482. (Abstract)
6. D. S. Manickam, A. Hirata, D. A. Putt, L. H. Lash, F. Hirata, D. Oupicky. "Overexpression of Bcl-2 as a proxy redox stimulus to enhance activity of non-viral redox-responsive delivery vectors." Biomaterials, 2008, 29, 2680-2688. ( Abstract )
7. Y. You, K. Kalebaila, S. L. Brock, D. Oupicky. "Temperature-Controlled Uptake and Release in PNIPAM-modified Porous Silica Nanoparticles." Chemistry of Materials, 2008, 20, 3354-3359. ( Abstract )
8. U. L. Rahbek, K. A. Howard, D. Oupicky, D. S. Manickam, M. Dong, A. F. Nielsen, F. Besenbacher, J. Kjems. "Intracellular siRNA and Precursor miRNA Trafficking Using Bioresponsive Copolypeptides." Journal of Gene Medicine, 2008, 10, 81-93. ( Abstract )
9. H. Zhang, R. Thomas, D. Oupicky, F. Peng. "Synthesis and characterization of new copper thiosemicarbazone complexes with an ONNS quadridentate system: cell growth inhibition, S-phase cell cycle arrest and proapoptotic activities on cisplatin-resistant neuroblastoma cells." Journal of Biological Inorganic Chemistry, 2008, 13, 47-55. ( Abstract )
10. Y. Z. You, Q. H. Zhou, D. S. Manickam, L Wan, G. Mao, D. Oupicky. "Dually Responsive Multiblock Copolymers via Reversible Addition-Fragmentation Chain Transfer Polymerization: Synthesis of Temperature- and Redox-Responsive Copolymers of Poly(N-isopropylacrylamide) and Poly(2-(dimethylamino)ethyl methacrylate)." Macromolecules, 2007, 40(24), 8617-8624. ( Abstract )
11. L. Wan, H. S. Bisht, Y. You, D. Oupicky, G. Mao. "Temperature-Reversible Ultrathin Films of N-Isopropylacrylamide Terpolymer Adsorbed at the Solid-Liquid Interface." Langmuir, 2007, 23, 12159-12166. ( Abstract )
12. Y. You, D. Soundara Manickam, Q. Zhou, D. Oupický. "Reducible poly(2-dimethyl aminoethyl methacrylate): Synthesis, cytotoxicity, and gene delivery activity." Journal of Controlled Release, 2007, 122, 217-225. ( Abstract )
13. Y. You, D. Soundara Manickam, Q. Zhou, D. Oupicky. "A versatile approach to reducible vinyl polymers via oxidation of telechelic oligomers prepared by RAFT polymerization." Biomacromolecules, 2007, 8, 2038-2044. ( Abstract )
14. M. Dong, K. A. Howard, D. Oupicky, H. Bisht, J. Kjems, F. Besenbacher. "Visualisation of thermal activated nanocarriers using in situ atomic force microscopy." Nanotechnology, 2007, 18(18), 185501 5(pp). ( Abstract )
15. Y. You, D. Oupicky. "Synthesis of temperature-responsive heterobifunctional block copolymers of PEG and PNIPAM." Biomacromolecules, 2007, 8, 98-105. ( Abstract )
16. J. Blacklock, H. Handa, D. Soundara Manickam, G. Mao, A. Mukhopadhy, D. Oupicky. "Disassembly of Layer-by-Layer Films of Plasmid DNA and Reducible TAT Polypeptide." Biomaterials, 2007, 28, 117-124. ( Abstract )
17. K. A. Howard, M. Dong, D. Oupicky, H. S. Bisht, C. Buss, F. Besenbacher, J. Kjems. "Nanocarrier Stimuli-Activated Drug Delivery." Small, 2007, 3, 54-57. ( Abstract )
18. J. Yuan, Y. You, X. Lu, O. Muzik, D. Oupicky, F. Peng. "Synthesis of Poly(APMA)-DOTA-64Cu conjugates for interventional radionuclide therapy of prostate cancer: Assessment of intra-tumoral retention by microPET imaging." Molecular Imaging, 2007, 6, 10-17. ( Abstract )
19. D. Soundara Manickam, D. Oupicky. "Multiblock reducible copolypeptides containing histidine-rich and nuclear localization sequences for gene delivery." Bioconjugate Chemistry, 2006, 17, 1395-1403. ( Abstract )
20. H. S. Bisht, D. Soundara Manickam, Y. You, D. Oupicky. "Temperature-Controlled Properties of DNA Complexes with Poly(ethylenimine)-graft-poly(N-isopropylacrylamide)." Biomacromolecules, 2006, 7, 1169-1178. ( Abstract )
21. Q. Zhou, D. L. Miller, R. C. Carlisle, L. W. Seymour, D. Oupicky. "Ultrasound-enhanced transfection activity of HPMA-stabilized DNA polyplexes with prolonged plasma circulation." Journal of Controlled Release, 2005, 106, 416-427. ( Abstract )
22. H. S. Bisht, L. Wan, G. Mao, D. Oupicky. "pH-Controlled association of PEG-containing terpolymers of N-isopropylacrylamide and 1-vinylimidazole." Polymer, 2005, 46, 7945-7952. ( Abstract )
23. M. L. Read, S. Singh, Z. Ahmed, M. Stevenson, S. Briggs, D. Oupicky, L. Barrett, U. Tirlapur, M. Berry, J. Preece, A. Logan, L. W. Seymour. "A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids." Nucleic Acids Research, 2005, 33(9), e86. ( Full text )
24. A. L. Parker, K.D. Fisher, D. Oupicky, M. L. Read, S. A. Nicklin, A. H. Baker, L. W. Seymour. "Enhanced gene transfer activity of peptide-targeted gene-delivery vectors." Journal of Drug Targeting, 2005, 13, 39-51. ( Abstract )
25. D. Soundara Manickam, H. S. Bisht, L. Wan, G. Mao, D. Oupicky. "Influence of TAT-peptide polymerization on properties and transfection activity of TAT/DNA polyplexes." Journal of Controlled Release, 2005,102, 293-306. ( Abstract )
26. D. Oupicky, T. Reschel, C. Konak, L. Oupicka. "Temperature controlled behavior of self-assembly gene delivery vectors based on complexes of DNA with poly(L-lysine)-graft-poly(N-isopropylacryl amide)." Macromolecules, 2003, 36, 6863-6872. ( Abstract )
27. M. L. Read, K. H. Bremner, D. Oupicky, N. K. Green, P. F. Searle, L. W. Seymour. "Vectors based on reducible polycations facilitate intracellular release of nucleic acids." Journal of Gene Medicine, 2003, 5, 232-245. ( Abstract )
28. D. Oupicky, A. L. Parker, L. W. Seymour. "Laterally stabilized complexes of DNA with linear reducible polycations: Strategy for triggered intracellular activation of DNA delivery vectors." Journal of the American Chemical Society, 2002, 124, 8-9. ( Abstract )
29. D. Oupicky, M. Ogris, K. A. Howard, P. R. Dash, K. Ulbrich, and L. W. Seymour. "Importance of lateral and steric stabilization of polyelectrolyte gene delivery vectors for extended systemic circulation." Molecular Therapy, 2002, 5, 463-472. ( Abstract )
30. C. Konak, T. Reschel, D. Oupicky, K. Ulbrich. "Thermally controlled association in aqueous solutions of poly(L-lysine) grafted with poly(N-isopropylacrylamide)." Langmuir, 2002, 18, 8217-8222. ( Abstract )
31. C. M. Ward, M. Pechar, D. Oupicky, K. Ulbrich and L. W. Seymour. "Modification of pLL/DNA complexes with a multivalent hydrophilic polymer permits folate-mediated targeting in vitro and prolonged plasma circulation in vivo." Journal of Gene Medicine, 2002, 4, 536–547. ( Abstract )
32. T. Reschel, C. Konak, D. Oupicky, L.W. Seymour , K. Ulbrich. "Physical properties and in vitro transfection efficiency of gene delivery vectors based on complexes of DNA with synthetic polycations." Journal of Controlled Release, 2002, 81 201-217. ( Abstract )
33. A. L. Parker, D. Oupicky, P. R. Dash, L. W. Seymour. "Methodologies for monitoring particle formation by self-assembly of DNA with poly(L-lysine)." Analytical Biochemistry, 2002, 302, 75-80. ( Abstract )
34. D. Oupicky, R. C. Carlisle, L.W. Seymour. "Triggered intracellular activation of disulfide crosslinked polyelectrolyte gene delivery complexes with extended systemic circulation in vivo." Gene Therapy, 2001, 8(9), 713-724. ( Abstract )
35. J. Kriz, D. Kurková, J. Dybal, D. Oupicky. "Cooperative interactions of unlike macromolecules: NMR study of ionic coupling of poly[2-(trimethylammonio)ethyl methacrylate chloride]-block-poly(N-(2-hydroxypropyl) methacrylamide) polycation with oligophosphates in D2O." Journal of Physical Chemistry A, 2000, 104, 10972-10985. ( Abstract )
36. C. Konak, D. Oupicky, V. Chytry, K. Ulbrich, M. Helmstedt. "Thermally controlled association in aqueous solutions of diblock copolymers of poly[N-(2-hydroxypropyl)methacrylamide] and poly(N-isopropylacryl-amide)." Macromolecules, 2000, 33, 5318-5320. ( Abstract )
37. D. Oupicky, K. A. Howard, C. Konak, P. R. Dash, K. Ulbrich, L.W. Seymour. "Steric stabilization of poly-L-lysine/DNA complexes by covalent attachment of semitelechelic poly[N-(2-hydroxypropyl)methacryl-amide]." Bioconjugate Chemistry, 2000, 11, 492-501. ( Abstract )
38. P. R. Dash, M. Read, K. Fisher, K. Howard, M. Wolfert, D. Oupicky, M. Šubr, J. Strohalm, K. Ulbrich and L. W. Seymour. "Decreased binding to proteins and cells of polymeric gene delivery vectors surface-modified with a multivalent hydrophilic polymer." Journal of Biological Chemistry, 2000, 275, 3793-3802. ( Full text )
39. D. Oupicky, C. Konak, K. Ulbrich, M. A. Wolfert, L. W. Seymour. "DNA delivery systems based on complexes of DNA with synthetic polycations and their copolymers." Journal of Controlled Release, 2000, 65, 149-171. ( Abstract )
40. M. L. Read, P. Dash, A. Clark, K. Howard, D. Oupicky, V. Toncheva, O. Alpar, E. H. Schacht, K. Ulbrich and L. W. Seymour. "Physicochemical and biological characterisation of an antisense oligonucleotide targeted against the bcl-2 mRNA complexed with cationic-hydrophilic copolymers." European Journal of Pharmaceutical Sciences, 2000, 10, 169-177. ( Abstract )
41. K. Ulbrich, J. Strohalm, D. Plocová, D. Oupicky, V. Šubr, J. Soucek, P. Poucková, J. Matoušek. "Poly[N-(2-hydroxypropyl)methacrylamide] conjugates of bovine seminal ribonuclease. Synthesis, physicochemical, and preliminary biological evaluation." Journal of Bioactive and Compatible Polymers, 2000, 15, 4-26. ( Abstract )
42. D. Oupicky, C. Konak, P. R. Dash, L. W. Seymour, K. Ulbrich. "Effect of albumin and polyanions on the structure of DNA complexes with polycation containing hydrophilic nonionic blocks." Bioconjugate Chemistry, 1999, 10(5), 764-772. ( Abstract )
43. M. A. Wolfert, P. R. Dash, O. Nazarova, D. Oupicky, L. W. Seymour, S. Smart, J. Strohalm, K. Ulbrich. "Polyelectrolyte vectors for gene delivery: Influence of cationic polymer on biophysical properties of complexes formed with DNA." Bioconjugate Chemistry, 1999, 10, 993-1004. ( Abstract )
44. D. Oupicky, C. Konak, K. Ulbrich. "DNA complexes with block and graft copolymers of N-(2-hydroxypropyl)methacrylamide and 2-(trimethylammonio)ethyl methacrylate." Journal of Biomaterials Science: Polymer Edition, 1999, 10, 573-590. ( Abstract )
45. D. Oupicky, C. Konak, K. Ulbrich. "Preparation of DNA complexes with diblock copolymers of poly[N-(2-hydroxypropyl) methacrylamide] and polycations." Materials Science & Engineering C-Biomimetic and Supramolecular Systems, 1999, 7, 59-65. ( Abstract )
46. D. Oupicky, K. Ulbrich, B. Rihova. "Conjugates of semitelechelic poly[N-(2-hydroxypropyl) methacrylamide] with enzymes for protein delivery." Journal of Bioactive and Compatible Polymers, 1999, 14, 213-231. ( Abstract )
47. V. Toncheva, M. A. Wolfert, P. R. Dash, D. Oupicky, K. Ulbrich, L. W. Seymour, E. H. Schacht. "Novel vectors for gene delivery formed by self-assembly of DNA with poly(L-lysine) grafted with hydrophilic polymers." Biochimica et Biophysica Acta, 1998, 1380, 354-368. ( Abstract )
48. G. Chen, R. G. Cooks, S. K. Jha, D. Oupicky, M. M. Green. "Block microstructural characterization of copolymers formed from fluorinated and non-fluorinated alkyl polyisocyanates using desorption chemical ionization mass spectrometry." International Journal of Mass Spectrometry and Ion Processes, 1997, 165/166, 391-404. ( Abstract )
49. P. Sysel, D. Oupicky. "Polyimide-polysiloxane block copolymers synthesized from 3-amino phenoxy terminated poly[oxy(dimethylsilyl)-2,4-phenylene(dimethylsilylene)]s." Polymer International, 1996, 40, 275-279 . ( Abstract )

Review Articles

50. D. Oupicky. "Design and development strategies of polymer materials for drug and gene delivery applications (Preface)" Advanced Drug Delivery Reviews, 2008, 60, 957. ( Issue )
51. D. Soundara Manickam, D. Oupicky. "Polyplex gene delivery modulated by redox potential gradients." Journal of Drug Targeting, 2006, 14, 519-526. ( Abstract )
52. D. Oupicky, H. S. Bisht, D. Soundara Manickam, Q. Zhou. "Stimulus-controlled delivery of drugs and genes." Expert Opinion on Drug Delivery, 2005, 2, 653-665. ( Abstract )
53. D. Oupicky, V. Diwadkar. "Stimuli-responsive gene delivery vectors." Current Opinion in Molecular Therapeutics, 2003, 5, 345-350. ( Abstract )
54. D. Oupicky, M. Ogris, L. W. Seymour. "Development of long-circulating polyelectrolyte complexes for systemic delivery of genes." Journal of Drug Targeting, 2002, 10, 93-98. ( Abstract )

Book Chapters

55. D. Oupický, Y. You, D. Soundara Manickam. "Redox-responsive polymer-based gene delivery systems." In Gene and Cell Therapy: Therapeutic Mechanisms and Strategies (3^rd edition), N. Smyth Templeton, Ed., Taylor&Francis, 2008.
56. D. Oupicky. "Subcellular Fate of Proteins and Nucleic Acids." In Biomaterials-based Delivery and Biocompatibility of Proteins and Nucleic Acids, R. I. Mahato, Ed., CRC Press, 2005.
57. D. Oupicky. "Use of HPMA Copolymers in Gene Delivery." In Polymeric Gene Delivery: Principles and Applications, M. M. Amiji, Ed., CRC Press, 2004.
58. L. W. Seymour, K. D. Fisher, N. K. Green, S. J. Hale, M. Lyons, V. Mautner, S. Nicum, D. Onion, D. Oupicky, M. Stevenson, K. Ulbrich "Adenovirus Retargeting and Systemic Delivery" In Human Gene Therapy: Present opportunities and future trends, G. M. Rubanyi and S. Yla-Herttuala, Eds., Ernst Schering Research Foundation Workshop Series, Volume 43, Springer Verlag, Heidelberg, 2003. ( Abstract )
59. D. Oupicky, M. L. Read, T. Bettinger. "Stabilization of polycation/DNA complexes by surface modification with hydrophilic polymers." In Nonviral Vectors for Gene Therapy (Methods in Molecular Medicine series), M. A Findeis, Ed., Humana Press, Totowa, Vol. 65, 61-78, 2001.
60. M. L. Read, T. Bettinger, D. Oupicky. "Methods for studying the formation and stability of polycation-DNA complexes." In Nonviral Vectors for Gene Therapy (Methods in Molecular Medicine series), M. A. Findeis, Ed., Humana Press, Totowa, Vol. 65, 131-148, 2001.